Interaction and ligand gating within Arabidopsis guard cell hormone-receptor anion-channel complex

Project Description

Stomata optimize plant CO2 uptake for photosynthesis and the associated water loss via transpiration. As a key site, stomatal anion channels are addressed by a multitude of stimuli, including drought stress and pathogen-derived ligands. We uncovered the molecular nature of the major plant plasma membrane anion channel family (SLAC/SLAHs) and the signaling pathways through which they are regulated. The SLAC/SLAH channels are characterized by slow voltage-dependent (S-type) activation. Under drought S-type anion channels in guard cells are stimulated by the stress hormone abscisic acid (ABA), which triggers a decrease in cell volume and turgor pressure and thereby causing stomatal closure.

Within the scope of our proposed project, we will focus on the guard cell expressed anion channel isoforms SLAC1 and SLAH3 that represent master switches within the ABA-stimulated signaling complex. We reconstituted the core ABA-signalosome, consisting of an ABA-receptor/phosphatase complex and an anion channel-activating kinase, in vitro as well as in the Xenopus oocyte expression system. In the absence of ABA, the protein phosphatase inhibits SLAC/SLAH-activating kinases and keeps the anion channels dephosphorylated and thus inactive. Upon perception of ABA, the phosphatase becomes inactivated by interaction with the cytosolic ABA receptors. Thereby the inhibition of protein kinases is relieved and anion channels are activated through phosphorylation. However, the knowledge about the spatio/temporal dynamics of interactions within the ABA-signalosome remains elusive.

Using super-resolution microscopy in combination with electrophysiological techniques we intent to quantify and to decipher the membrane-delimited ABA signaling complex with spatio/temporal resolution. Similar to rafts in animals, membrane lipid nanodomains constitute signaling platforms in the plasma membrane of plants. Upon ABA stimulation the anion channel SLAH3 physically interacts with a protein kinase/phosphatase pair in these sterol-rich nanodomains. To better resolve the spatio/temporal and dynamic distribution of SLAH3 and its interacting partners we will utilize super-resolution microscopy (PALM and dSTORM), FRET/FLIM and single molecule tracking techniques. Very recently, we could show that the two members SLAH1 and 4 of the SLAC/SLAH anion channel family are electrically silent as homomers. When co-expressed with SLAH3, however, SLAH1 modulates the activation mechanism and the selectivity of SLAH3. In contrast, the formation of SLAH4/SLAH3 heteromers suggests an electrically silent state. We will follow the interaction of SLAH3 with its modulating beta-subunits using super-resolution microscopy and electrophysiology. Our approach will gain the first insight into the dynamics of i) drought hormone ABA recognition by the guard cell receptor complex, ii) the complex addressing the S-type anion channel(s), and iii) functional heteromeric assembly of SLAC/SLAH-type subunits.

Title Year Authors Journal Links
A Tandem Amino Acid Residue Motif in Guard Cell SLAC1 Anion Channel of Grasses Allows for the Control of Stomatal Aperture by Nitrate 2018 Schäfer, N., Maierhofer, T., Herrmann, J., Jorgensen, M.E., Lind, C., von Meyer, K., Lautner, S., Fromm, J., Felder, M., Hetherington, A.M., Ache, P., Geiger, D., and Hedrich, R. Curr Biol More
The receptor-like pseudokinase GHR1 is required for stomatal closure 2018 Sierla, M., Horak, H., Overmyer, K., Waszczak, C., Yarmolinsky, D., Maierhofer, T., Vainonen, J. P., Denessiouk, K., Salojarvi, J., Laanemets, K., Toldsepp, K., Vahisalu, T., Gauthier, A., Puukko, T., Paulin, L., Auvinen, P., Geiger, D., Hedrich, R., Kollist, H., and Kangasjarvi, J. Plant Cell More
Sulfate is incorporated into cysteine to trigger ABA production and stomatal closure 2018 Batool, S., Uslu, V. V., Rajab, H., Ahmad, N., Waadt, R., Geiger, D., Malagoli, M., Xiang, C.-B., Hedrich, R., Rennenberg, H., Herschbach, C., Hell, R., and Wirtz, M. Plant Cell More
Silent S-Type Anion Channel Subunit SLAH1 Gates SLAH3 Open for Chloride Root-to-Shoot Translocation 2016 Cubero-Font, P., Maierhofer, T., Jaslan, J., Rosales, M. A. , Espartero, J., Diaz-Rueda, P. , Mueller, H. M. , Huerter, A.-L., Al-Rasheid, K. A. S., Marten, I. , Hedrich, R. , Colmenero-Flores, J. M. , Geiger, D. Curr Biol More
Biology of SLAC1-type anion channels - from nutrient uptake to stomatal closure 2016 Hedrich, R., and Geiger, D. New Phytolog More
Abscisic acid controlled sex before transpiration in vascular plants 2016 McAdam, S.A., Brodribb, T.J., Banks, J.A., Hedrich, R., Atallah, N.M., Cai, C., Geringer, M.A., Lind, C., Nichols, D.S., Stachowski, K., Geiger, D., and Süssmilch, F.C. PNAS USA More
A Single-Pore Residue Renders the Arabidopsis Root Anion Channel SLAH2 Highly Nitrate Selective 2014 Maierhofer, T., Lind, C., Huttl, S., Scherzer, S., Papenfuss, M., Simon, J., Al-Rasheid, K. A., Ache, P., Rennenberg, H., Hedrich, R., Muller, T. D., and Geiger, D. Plant Cell More
Site- and kinase-specific phosphorylation-mediated activation of SLAC1, a guard cell anion channel stimulated by abscisic acid 2014 Maierhofer, T., Diekmann, M., Offenborn, J. N., Lind, C., Bauer, H., Hashimoto, K., Al-Rasheid, K. A., Luan, S., Kudla, J., Geiger, D., and Hedrich, R. Sci Signal More
Arabidopsis nanodomain-delimited ABA signaling pathway regulates the anion channel SLAH3 2013 Demir, F., Horntrich, C., Blachutzik, J. O., Scherzer, S., Reinders, Y., Kierszniowska, S., Schulze, W. X., Harms, G. S., Hedrich, R., Geiger, D., and Kreuzer, I. Proc Natl Acad Sci USA More
Anion channels: master switches of stress responses 2012 Roelfsema, M. R., Hedrich, R., and Geiger, D. Trends Plant Sci More
Molecular Evolution of Slow and Quick Anion Channels (SLACs and QUACs/ALMTs) 2012 Dreyer, I., Gomez-Porras, J. L., Riano-Pachon, D. M., Hedrich, R., and Geiger, D. Front Plant Sci More
Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1 2011 Geiger, D., Maierhofer, T., Al-Rasheid, K. A., Scherzer, S., Mumm, P., Liese, A., Ache, P., Wellmann, C., Marten, I., Grill, E., Romeis, T., and Hedrich, R. Sci Signal More
Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities 2010 Geiger, D., Scherzer, S., Mumm, P., Marten, I., Ache, P., Matschi, S., Liese, A., Wellmann, C., Al-Rasheid, K. A., Grill, E., Romeis, T., and Hedrich, R. Proc Natl Acad Sci USA More
Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair 2009 Geiger, D., Scherzer, S., Mumm, P., Stange, A., Marten, I., Bauer, H., Ache, P., Matschi, S., Liese, A., Al-Rasheid, K. A., Romeis, T., and Hedrich, R. Proc Natl Acad Sci USA More
Cytosolic abscisic acid activates guard cell anion channels without preceding Ca2+ signals 2005 Levchenko, V., Konrad, K. R., Dietrich, P., Roelfsema, M. R., and Hedrich, R. Proc Natl Acad Sci USA More
ABA depolarizes guard cells in intact plants, through a transient activation of R- and S-type anion channels 2004 Roelfsema, M. R., Levchenko, V., and Hedrich, R. Plant J More