FRET-based monitoring of Adhesion class G protein-coupled receptor activity

Project Description

G protein-coupled receptors (GPCR) of the Adhesion class are a large group of transmembrane receptor molecules that possesses molecular moieties from adhesion proteins and GPCR at equal shares. It is still unknown, which modality Adhesion-GPCR perceive and how they transduce a signal across the membrane. A prevailing hypothesis suggests that changes in distance between the juxtamembraneously positioned GPCR autoproteolysis-inducing (GAIN) domain of Adhesion-GPCR and their heptahelical transmembrane (7TM) unit through mechanical stimuli regulate metabotropic receptor activity (distance hypothesis). The GAIN-7TM domain tandem is harboured by all 33 known Adhesion-GPCR homologs with one exception and is thus suspected to play a paramount role in this novel mechanosensing principle.

Confirmation of the distance hypothesis requires (i) determination of the interaction between the GAIN and 7TM domains of Adhesion-GPCR, (ii) testing whether proximity between GAIN and 7TM domains quantitatively affects signal output of Adhesion-GPCR, and (iii) physiological receptor activity.

This proposal is motivated by the distance hypothesis and will use a combination of optical and physiological approaches to investigate Adhesion-GPCR function. First, selected Adhesion-GPCR (i.e. Latrophilins, CELSR, GPR56) will be adapted for FRET measurements of GAIN-7TM interactions to determine spatial interdomain changes over time when the receptors are challenged with cognate ligands, mechanical stimulation and a combination of both. Resulting changes in intramolecular receptor distances can subsequently be correlated with cellular readouts in vitro and in vivo using established assays for Drosophila melanogaster.

Second, Adhesion-GPCR are involved in a wide scope of human pathologies, where their disruption results in grave organ dysfunction. We will use Adhesion-GPCR FRET sensors to screen a panel of human mutations that map to the extracellular portions of the receptors (including GAIN and 7TM domains) and investigate their impact on distance changes between both domains. Third, we will assess whether relaxation of mechanical tension within the extracellular domain of Adhesion-GPCR results in changes of physiological activity in vivo. This will be tested by the introduction of spacer domains of different lengths between adhesion folds and the GAIN domain. For this set of experiments we will concentrate on Latrophilin/dCIRL, an Adhesion-GPCR in Drosophila, whose function in mechanosensation was recently determined by my group. The effect of stepwise tensile loading and relaxation of Latrophilin/dCIRL will be evaluated through in vivo assays of mechanical qualities.

Obtaining experimental evidence to substantiate the distance hypothesis will fundamentally contribute a mechanistic concept of Adhesion-GPCR signaling. This will lay the foundations to understand the common functional denominator among Adhesion-GPCR, which is still at large, and to manipulate Adhesion-GPCR function.

Title Year Authors Journal Links
Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix 2019 Scholz, N., Ehmann, N., Sachidanandan, D., Imig, C., Cooper, B. H., Jahn, O., Reim, K., Brose, N., Meyer, J., Lamberty, M., Altrichter, S., Bormann, A., Hallermann, S., Pauli, M., Heckmann, M., Stigloher, C., Langenhan, T., and Kittel, R. J. J Cell Biol More
Parallel genomic engineering of two Drosophila genes using orthogonal attB/attP sites 2018 Blanco-Redondo, B., and Langenhan, T. G3 More
Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons 2017 Scholz, N., Guan, C., Nieberler, M., Grotemeyer, A., Maiellaro, I., Gao, S., Beck, S., Pawlak, M., Sauer, M., Asan, E., Rothemund, S., Winkler, J., Prömel, S., Nagel, G., Langenhan, T., and Kittel, R. J. eLife More
Adhesion G protein-coupled receptors in nervous system development and disease 2016 Langenhan, T., Piao, X. and Monk, K. R. Nat Rev Neurosci More
Control of adhesion GPCR function through proteolytic processing 2016 Nieberler, M., Kittel, R. J., Petrenko, A. G., Lin, H. H., Langenhan, T. Handb Exp Pharmacol More
Adhesion GPCRs as a putative class of metabotropic mechanosensors 2016 Scholz, N., Monk, K. R., Kittel, R. J., Langenhan, T. Handb Exp Pharmacol More
Model Organisms in G Protein-Coupled Receptor Research 2015 Langenhan, T., Barr, M. M., Bruchas, M. R., Ewer, J., Griffith, L. C., Maiellaro, I., Taghert, P. H., White, B. H., and Monk, K. R. Mol Pharmacol More
Adhesion G Protein-Coupled Receptors: From In Vitro Pharmacology to In Vivo Mechanisms 2015 Monk, K. R., Hamann, J., Langenhan, T., Nijmeijer, S., Schöneberg, T., and Liebscher, I. Mol Pharmacol More
The Adhesion GPCR Latrophilin/CIRL Shapes Mechanosensation 2015 Scholz, N., Gehring, J., Guan, C., Ljaschenko, D., Fischer, R., Lakshmanan, V., Kittel, R. J., and Langenhan, T. Cell Reports More
International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G Protein-Coupled Receptors 2015 Hamann, J., Aust, G., Arac, D., Engel, F. B., Formstone, C., Fredriksson, R., Hall, R. A., Harty, B. L., Kirchhoff, C., Knapp, B., Krishnan, A., Liebscher, I., Lin, H.-H., Martinelli, D. C., Monk, K. R., Peeters, M. C., Piao, X., Prömel, S., Schöneberg, T., Schwartz, T. W., Singer, K., Stacey, M., Ushkaryov, Y. A., Vallon, M., Wolfrum, U., Wright, M. W., Xu, L., Langenhan, T., and Schiöth, H. B. Pharmacol Rev More
Channelrhodopsin-2-XXL, a powerful optogenetic tool for low light applications 2014 Dawydow, A., Gueta, R., Ljaschenko, D., Ullrich, S., Hermann, M., Ehmann, N., Gao, S., Fiala, A., Langenhan, T., Nagel, G., Kittel, R.J. Proc Natl Acad Sci USA More
Sticky signaling - Adhesion class G protein-coupled receptors take the stage 2013 Langenhan, T., Aust, G., and Hamann, J. Sci Signal More
Matching structure with function: the GAIN domain of Adhesion-GPCR and PKD1-like proteins 2013 Prömel, S., Langenhan, T., and Arac, D. Trends Pharmacol Sci More
Characterization and functional study of a Cluster of four highly conserved orphan adhesion-GPCR in mouse 2012 Prömel, S., Waller-Evans, H., Dixon, J., Zahn, D., Colledge, W. H., Doran, J., Carlton, M. B. L., Grosse, J., Schöneberg, T., Russ, A. P., and Langenhan, T. Dev Dyn More
The GPS Motif Is a Molecular Switch for Bimodal Activities of Adhesion Class G Protein-Coupled Receptors 2012 Prömel, S., Frickenhaus, M., Hughes, S., Mestek, L., Staunton, D., Woollard, A., Vakonakis, I., Schöneberg, T., Schnabel, R., Russ, A. P., and Langenhan, T. Cell Rep More
Dissecting signaling and functions of adhesion G protein-coupled receptors 2012 Arac, D., Aust, G., Calebiro, D., Engel, F. B., Formstone, C., Goffinet, A., Hamann, J., Kittel, R. J., Liebscher, I., Lin, H.-H., Monk, K. R., Petrenko, A., Piao, X., Prömel, S., Schiöth, H. B., Schwartz, T. W., Stacey, M., Ushkaryov, Y. A., Wobus, M., Wolfrum, U., Xu, L., and Langenhan, T. Ann NY Acad Sci More
The orphan adhesion-GPCR GPR126 is required for embryonic development in the mouse 2010 Waller-Evans, H., Prömel, S., Langenhan, T., Dixon, J., Zahn, D., Colledge, W. H., Doran, J., Carlton, M. B. L., Davies, B., Aparicio, S. A. J. R., Grosse, J., and Russ, A. P. PLoS ONE More
Latrophilin signaling in tissue polarity and morphogenesis 2010 Langenhan, T., and Russ, A. P. Adv Exp Med Biol More
Latrophilin signaling links anterior-posterior tissue polarity and oriented cell divisions in the C. elegans embryo 2009 Langenhan, T., Prömel, S., Mestek, L., Esmaeili, B., Waller-Evans, H., Hennig, C., Kohara, Y., Avery, L., Vakonakis, I., Schnabel, R., and Russ, A. P. Dev Cell More
Solution structure and sugar-binding mechanism of mouse latrophilin-1 RBL: a 7TM receptor-attached lectin-like domain 2008 Vakonakis, I., Langenhan, T., Prömel, S., Russ, A., and Campbell, I. D. Structure More